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KMID : 1005220100250020093
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Journal of Embryo Transfer
2010 Volume.25 No. 2 p.93 ~ p.96
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Comparison of Mechanical and Enzymatic Methods for the Isolation of Bovine Ovarian Follicles
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Lim Hyun-Joo
Kim Dong-Hun
Im Gi-Sun
Lim Jeong-Mook
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Abstract
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The isolation of preantral follicles from the ovaries of bovine was performed under mechanical and enzymatic methods. A significant increase in the total number of follicles retrieved was detected when tissue chopper was used. Micro-dissection could supply good quality, larger sized follicles (400 to ) but with the lowest yield (). The isolated preantral and early antral follicles were cultured for 14 days. Follicles isolated by the mechanical method had a greater growth during a culture period than follicles collected enzymatically. Morphologically normal bovine oocytes from early antral follicles after 14 days culture were 59.6% after culture and after 24 h of maturation culture, 12.9% of in vitro-grown oocytes reached the second metaphase. In conclusion, this study showed that mechanical method can be used effectively to isolate intact preantral follicles from bovine ovaries.
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KEYWORD
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bovine, follicles, isolation
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